Reprogramming

Generation of Induced Pluripotent Stem Cells

Reprogramming enables the conversion of differentiated somatic cells into induced pluripotent stem cells, providing a platform for disease modeling, drug discovery, and regenerative medicine.

Reprogramming workflow with PBMC (Generali et al.,2025)

We generate induced pluripotent stem cell lines from patient or control samples using non integrating reprogramming approaches. The most common starting materials are peripheral blood mononuclear cells (PBMCs) and dermal fibroblasts, although additional cell types may be considered depending on the project.

Each reprogramming project includes:

• Transient overexpression of defined reprogramming factors (KOS, Klf4, c-Myc).
• Manual colony picking
• Expansion of reprogrammed iPSC lines
• Quality control
• Cryopreservation of established lines

To ensure high-quality cell lines, we perform comprehensive quality control, including:

• Assessment of pluripotency marker expression on RNA and protein level
• Evaluation of differentiation potential by Trilineage Differentiation
• SNP arary analysis to assess genomic integrity
• Mycoplasma testing
• Morphology assessment

Every research project has unique requirements. Therefore, our services can be combined into individualized service packages optimized to the specific needs of each project.