Gene Editing

CRISPR/Cas9 Gene Editing in Human iPSC Lines

The Core Facility offers CRISPR/Cas9 based gene editing to generate customized human iPSC models for basic and translational research. We support projects involving the introduction, correction, or disruption of genetic variants, as well as the generation of isogenic control cell lines.

Our services include:

• Guide RNA design
• CRISPR/Cas9 mediated genome editing
• Clonal isolation and expansion of edited iPSC lines
• Comprehensive quality control

Editing outcomes and iPSC quality are assessed:

• Sanger sequencing including off target PCR
• Pluripotency marker expression by flow cytometry
• Genomic integrity analysis by SNP array
• Mycoplasma testing
• Routine evaluation of cell morphology.

Edited iPSC lines can subsequently be used for downstream applications, including directed differentiation and disease modeling.

We collaborate closely with the UZH CRISPR Screening Hub on all CRISPR-related applications, combining complementary expertise to provide comprehensive support (https://www.crisprhub.uzh.ch/en.html).