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This forum is focused on discussing and developing the procedures, protocol and progression of young researchers working with iPSCs in Zürich.
Objectives:
PhD, UZH - Kinderspital Zürich, Division of Metabolism. Working on patient-derived iPS cells to model rare inborn errors of metabolism. Works with “rosette-type” differentiation to neural lineage (cortical, striatal) and 3D differentiation approaches. Also validating the patient-specific mechanisms caused by a monogenic mutation, which involves CRISPR designed isogenic controls and deep phenotype characterization. These culminate in a comprehensive disease model to study and better understand pathomechanisms related to rare inborn errors of metabolism.
Key Interests: Neural differentiation, neurodevelopment, rare diseases, Immunocytochemistry-immunofluorescence, disease modelling, CRISPR
Postdoc, UZH – Insititute of Pharmacology and Toxicology. Researching how circadian rhythms emerge when cells differentiate, e.g. from iPSCs to fibroblasts/neurons. Current problem: (1) Reproducibility in differentiation protocols of human iPSCs to fibroblasts. (2) Long term culture of human iPSC-derived neurons (by NGN2 protocol) in normal cell culture plate and on multielectrode array. (3) Infection of human iPSC-derived neurons with lentiviral reporter.
Key Interests: iPSC Culture, multi-line fibroblast culturing, multi-species fibroblasts
PhD, UZH- Institute of medical Genetics. Investigating a distinct Ciliopathy called Joubert Syndrome, a Mendelian disorder caused by dysfunction of primary cilia. Generates isogenic iPSC lines harbouring knockout in selected cilia-related genes using CRISPR-cas9. Establishes a Joubert Syndrome brain model by differentiating edited iPSC lines into cerebral organoids and determines resulting ciliary and cellular phenotypes. Also testing individual patient sequence variants in Joubert Syndrome genes using human neural stem cells and organoids.
Key Interests: Brain Organoids, CRISPR-cas9, neural differentiation, Immunocytochemistry-immunoflourescence, confocal microscopy, Transfection, Sequencing
Postdoctoral Fellow, UZH – Institute for Regenerative Medicine (IREM). Working on reprogramming human blood cells into iPSC, differentiation into smooth muscle cells, endothelial cells and cardiomyocytes (for Tissue Engineering). Focused on the generation of human iPSC/iPSC-derived cell without using serum or xenogenic reagents for clinical applications. Using microRNA-responsive synthetic mRNAs (RNA switches) to purify hiPSC-derived cells in collaboration with CiRA, Kyoto University.
Key Interests: iPSC Culture, mRNA, cardiomyocyte differentiation
PhD, ETH – Institute for Biomedical Engineering. Works with human iPSC-derived glutamatergic cortical neurons, obtained by overexpression of the neurogenin 2 (Ngn2) gene. Interests surround the elementary functions of small assemblies of neurons. Consequently, building small and highly controlled in vitro neuronal networks in Polydimethylsiloxane microstructures, which are then stimulate and recorded using microelectrode arrays (MEAs). This has been characterized in rat primary neurons and current projects attempt to demonstrate the technology in iPSC-derived neurons.
Key Interests: Multielectrode array recording, microfabrication (soft lithography, microcontact printing), signal processing, surface chemistry, Ngn2 induced neurons culture, rat primary cortical and hippocampal neuron culture
PhD student, ETH Zurich - Department of Biosystems Science and Engineering. In collaboration with the University Hospital of Zurich, Jihyun’s current research focuses on electrophysiological characterization of human iPSC-derived cardiomyocytes and their response to drugs using high-density microelectrode arrays (HD-MEAs). The data analysis scheme encompasses deep learning and feature-extraction for clinical translation of the study. The project aims at identifying genotype-phenotype correlations and genotype-specific treatment in channelopathies and arrhythmogenic cardiomyopathies.
Key Interests: Microelectrode array recording and stimulation, machine learning, signal processing, iPSC and cardiomyocyte culture
Currently studying for a BSc in Biochemistry at the UZH. Interests surround
iPSC handling and learning more about their application to biological research. Maximilian is hoping to gain experience with iPSCs in Japan and in particular the T-CiRA project. He is currently seeking lab experience in iPSC research facilities.
